WebDownload scientific diagram Functional evaluation of the G77A mutant. (A) A macroscopic current recording of the G77A mutant was elicited by a pH jump from 8.0 to 3.0. (B) Amplitude-normalized ... WebDec 20, 2024 · Here, we present the atomic resolution crystallographic structure, the function, and the ion-binding properties of the KcsA mutants, G77A and G77C, that stabilize the 2,4-ion-bound configuration (i.e., water, K + , water, K + -ion-bound configuration) of the K + channel's selectivity filter. A full functional and thermodynamic characterization of the …
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WebSh. Sheet Standard Origin Repl. f. Repl. by Proved User date Status change date name We reserve all rights in this document and in the informa- tion contained therein. WebSep 22, 2024 · The optimum pH of mutants V75A, G77A, N94A, A164S and F243A were shifted from 8.0 to 6.0, and mutant V75A has the highest pH stability and activity at acidic conditions than other mutants, which was more suitable for industrial application to manufacture gallic acid. This study was of great significance to promote the … cure cystic fibrosis miami foundation
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