Promoterless vector
WebThe promoterless pGL4.10 [ luc 2] firefly vector was transactivated 11- to 12-fold with corticosterone or with estradiol and corticosterone. This is a 4-fold greater induction than that observed ... Web안녕하세요, 돼지 체세포 (endothelial cell)에서 원하는 유전자를 발현시키기 위한 공부를 하고 있는 석사 재...
Promoterless vector
Did you know?
WebThe reporter vectors for GFP include a promoterless GFP vector for monitoring the expression of cloned promoters/enhancers in mammalian cells and a series of six vectors for creating fusion protein to either the N or C terminus of GFP. MeSH terms 3T3 Cells Animals Base Sequence CHO Cells Cell Line Cricetinae Gene Expression* Genetic Markers http://xmjsci.com/class/4?brand=2&page=10
WebJan 22, 2014 · A Promoterless Reporter System to Study the Mis-expression Effect Caused by Promoter Activity, Spacer and Configuration. To precisely monitor the transcript level outputted by any cis regulatory element in rice … WebThis material is available to academics and nonprofits only. Backbone Vector backbone na (Search Vector Database) Backbone size w/o insert (bp) 3900 Vector type Mammalian Expression, Cre/Lox Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert
WebThese expression vectors are designed to accommodate large inserts of 7-8 kb, and our promoterless vector can accommodate up to 9 kb. NOTE: Viral titers typically decrease with large inserts. Subscribe to Lentiviral Expression Vectors Lentiviral Promoterless Expression Vector Lentiviral Expression Vector (Hygro) WebMar 11, 2024 · Two AAV8 vectors were used: a promoterless vector with two arms of homology for the albumin locus, and a vector carrying the CRISPR/SaCas9 and the …
WebApr 12, 2024 · An accurate visual reporter system to assess homology-directed repair (HDR) is a key prerequisite for evaluating the efficiency of Cas9-mediated precise gene editing. …
WebDec 4, 2012 · The lack of highly active endogenous promoters to drive the expression of transgenes is one of the main drawbacks to achieving efficient transformation of many microalgal species. Using the model chlorophyte Chlamydomonas reinhardtii and the paromomycin resistance APHVIII gene from Streptomyces rimosus as a marker, we have … nether alderley millWebProduct Details Lentiviral vector-promoterless, luciferase reporter can be used to monitor your promoter activity in vitro and in vivo. Clone your promoter of interest upstream of the firefly luciferase reporter, then co-transfect with EZ-LentiPACK Packaging System into packaging cells, such as 293T to produce VSV-G pseudotyped lentivirus. nether alderley primary school macclesfieldWebDownload Table Promoterless Reporter Vectors from publication: Analysis of Gene Promoter Regulation by Tumor Suppressor Genes Gene expression is controlled at … nether alderley primaryWebOn-demand reporter vectors are the next generation of promoter reporters. With these reporter systems, you can compensate effectively for reporter background without compromising your assay’s signal intensity. Low background and a bright signal are no longer mutually exclusive. More Overview The next generation of promoter reporters nether alderley primary school term datesWebpAAV-MCS Promoterless Expression Vector Product Details Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a … nether alderleyWebA version of the targeted trapping vector without transmembrane do- main (TM) (pTT0, pTT1, and pTT2), which was used for targeted trapping of genes encoding cytosolic … itw creative worksWebApr 12, 2024 · Reporter vector was designed containing an 800-bp left homologous arm cover the exon 8, 9, 10, 11 and 12 upstream from the stop codon, and a 500-bp right homologous arm downstream of the stop codon. ( B) Validation intracellular indels efficiency and indels pattern of sgRNA3 targeting exon 12 of porcine GAPDH by TIDE … itwcp shop