Smad3 western blot
WebbHuman Phospho-Smad2/3 (S465/S467) Antibody Summary Species Reactivity Human Specificity Detects human Smad2 and Smad3 when dually phosphorylated at S465 and …
Smad3 western blot
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WebbSMAD3 Antibodies. Antibodies that detect SMAD3 can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, … WebbWestern blot assay The purified cells treated with MENK and untreated cells were lysed in cell disruption buffer (mirVana TMPARIS RNA and Native Protein Purification Kit; Invitrogen Life Technology, CA). Protein samples (40 mg) were subjected to electrophoretic analysis through 10% SDS-PAGE43 and subsequently transferred
Webb18 aug. 2024 · (A) Differentiated E11 podocytes were pretreated with Smad3 inhibitor (SIS3, 10 µM) and then stimulated with TGF-β (5 ng/mL) for 8 h. Jag1 mRNA was analyzed by real-time quantitative PCR; (B) Podocytes were pretreated with Y-27632 (10 µM) before stimulation with TGF-β (5 ng/mL) for 30 min. Cell lysates were subjected to Western … WebbIt is well known that activation of the Smad pathway and its subsequent nuclear translocation are critical steps in TGF-β1-mediated renal fibrosis in DN. In our study, TGF-β1, Smad2, Smad3, and Smad4 expression was detected by Western blot (Figure 7E–G), while their mRNA levels were detected by RT-PCR (Figure 7A–D).
WebbReceptor-regulated Smads (R-Smads), Smad2 and Smad3, are directly phosphorylated and activated by ALK5. Phosphorylation occurs at C-terminal SS X S motifs and promotes the … Webb3 feb. 2024 · Smad2/3 Antibody (A-3) is a mouse monoclonal IgG 2b κ, cited in 13 publications, provided at 200 µg/ml specific for an epitope mapping between amino …
http://yxbwk.njournal.sdu.edu.cn/CN/10.6040/j.issn.1671-7554.0.2024.1226
WebbIt was further verified in relative cell lines by real-time quantitative polymerase chain reaction and Western blotting (WB). Adenocarcinoma cell lines were transfected with si-NC and si- AHNAK2 by lipofectamine 3000 and treated with or without TGF-β 1, and cell migration and invasion were detected by wound-healing and transwell assays. life insurance policy settlement optionsWebbProtein levels of endogenous Smad3 were analyzed by Western blot and quantified on the right. Smad3 level in the KO cells at time “0” was defined as 1.0. (B) Specific knockdown … life insurance policy selling scriptWebb15 jan. 2003 · (A) Western blot analysis of phosphorylation of endogenous Smad2 in HL-60 cells untreated or treated with 100 nM Vit D 3 for 24 hours. (B) Immunohistochemical … life insurance policy shortestWebbSIS3 free base is a potent and selective inhibitor of Smad3 phosphorylation. SIS3 free base inhibits the myofibroblast differentiation of fibroblasts by TGF-β1. SIS3 free base does not affect the phosphorylation of Smad2 . For research use only. We do not sell to patients. SIS3 free base Chemical Structure CAS No. : 1009104-85-1 or Bulk Inquiry life insurance policy status onlineWebbImmunohistochemistry, Western blot and RT-PCR were used to detect IL-37 and Smad3. Results (1) Animal ... Western blot, RT-PCR all showed IL-37 and Smad3 in the experimental group was the highest, followed by drug group, the … mcroberts securityWebbWestern blot analysis also confirmed that Smad3 / mice failed to express Smad3 in the kidney after either sham ischemia or renal ischemia, thereby confirm- ing the deficiency … life insurance policy single 40Webb4 apr. 2024 · Methods and Results: Western blot data showed that APE1 and SMAD3 were highly expressed in EAC cell lines. APE1 silencing reduced SMAD3 nuclear expression … life insurance policy search illinois