WebbThe Uracil-DNA Glycosylase derived from Gadus morhua has all the attributes of the enzyme derived from E. coli with the added benefit of being heat-labile. It is completely … Webb26 aug. 2024 · 使用udg酶可去除含 du 的扩增产物气溶胶污染。 4、R Nase抑制剂RNase inhibitor 翌圣RNase inhibitor是以可溶形式在大肠杆菌中表达纯化的重组鼠源RNase抑制 …
Guidelines for comparing SYBR Green master mixes
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What is UNG/UDG? Thermo Fisher Scientific - US
Webb31 mars 2024 · UDG activation (1 cycle, 2 min, 50 °C) and polymerase activation (1 cycle, 10 min, 94 °C) were followed by 40 cycles of denaturation (15 s, 95 °C) and anneal/extend (1 min, 60 °C). The PCR reactions contained 500 nM of each primer, 0.5 × PowerUP SYBR Green Master Mix (2×) (A25742, Applied Biosystems), and 20 ng of cDNA in a final … WebbPlatinum® SYBR® Green qPCR SuperMix-UDG Cat. no. 11733-038 Size: 100 reactions Cat. no. 11733-046 Size: 500 reactions Store at –20°C Description Platinum ® SYBR Green … WebbThermo Scientific Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil … Thermo Scientific Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of … Thermo Scientific Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes how do i apply for spousal benefits