Thp-1 macrophage-like cells
WebFeb 3, 2024 · Adding THP-1 macrophages to the culture would further down-regulate the cell viability, as evidenced by over 50% decrease of the cell proliferation at 8 h after co-culturing (Fig. 1A). WebImmunomodulatory activity of a novel polysaccharide extracted from Huangshui on THP-1 cells through NO production and increased IL-6 and TNF-α expression. Author links open …
Thp-1 macrophage-like cells
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WebThe invention discloses a construction method of a mononuclear macrophage model based on HIV-1 (human immunodeficiency virus type 1) mass infection duplication, which comprises the following steps: designing a pair of identification sequences for SAMHD1 gene CDS1, and constructing a TALEN plasmid pair; transfecting the TALEN plasmid pair … WebThe activity of AuNDs was evaluated using macrophage functions and antioxidant response in the human macrophage cell line THP-1. ... Nrf2 localizes within the cytoplasm and …
WebFigure 1. (A) Experimental outline for comparative kinome profiling of undifferentiated (monocytic) and differentiated (macrophage-like) THP-1 cells. Cells were metabolically … WebIn general, THP-1 cells exhibit a large, round, single-cell morphology. The cells were derived from the peripheral blood of a 1-year-old human male with acute monocytic leukemia. …
WebWe also found the same trends for CARINH and IL18BP levels upon the knockdown and overexpression of IRF1 in THP-1 cells (Fig. 5j, k), again supporting the existence of a … WebIn the coculture system, THP-1 cells were seeded on six-well plates at a density of 1×10 6 cells per well and treated with 10 µM PMA for 48 h to form THP-1 macrophages (THP-M …
WebTo examine the impact of macrophage-secreted factors on cell behavior, 12Z cells on all three substrates were treated with conditioned media from differentiated THP-1 (an …
WebJan 7, 2024 · We next assessed the functionality of NK-4-treated THP-1 macrophages by analyzing cytokine production. NK-4 dose-dependently increased THP-1 macrophage secretion of the proinflammatory cytokine TNF-α after 3 days of culture (Fig. 1b). At 4 μM NK-4, TNF-α secretion increased by 36-fold compared to control cells (Fig. 1b). NK-4 did … smile in a day cost ukWebMar 24, 2024 · To differentiate U937 and THP-1 cells into active macrophage-like cells, the cells were resuspended in culture medium containing 0.1 μM 1α, 25-dihydroxyvitamin D 3 … risora s40ztsxp-fWebApr 27, 2010 · Undifferentiated, monocytic THP-1 cells are sensitive to killing by purified Stx1, while differentiated, macrophage-like THP-1 cells are relatively refractory to the cytotoxic action of the toxin, with only approximately 30% of cells undergoing apoptosis. risora s36ytsxs-fWebJan 13, 2024 · FIGURE 1. Figure 1. The morphologies of THP-1 monocytes and their derived macrophage-like cells at resting stage (M0) and immature dendritic-like cells (iDC) can … risora s22ytsxs-fWebLoss of ME-1 in THP-1 cells was also associated with reduced efferocytosis. ME-1 is a target gene of NRF2 (nuclear factor erythroid 2–related factor 2), and the authors have … risora s56ytsxp-fsmile in a day folkestoneWebPathway enrichment analysis of genes related to tumor monocyte/macrophage cell communication also suggested an ... The solid part of ACP is composed mainly of PE, stellate cells, and whorl-like cell clusters, which reflects ... was added. Half of the medium was changed every 48 hours. THP-1 cells and PBMCs were cultured in 1640 medium ... smile improvement poway